Main Article Content
Aims: The current study targets the achievement of a reliable process for the determination of PAH contents in kola nuts for better appreciation of the risks incurred from the consumption of such food products.
Study Design: Kolanuts collected from two big storage centers were analyzed after the validation of the proposed analytical method.
Place and Duration of Study: Central Laboratory for Food Hygiene and Agro-Industry, LANADA in Abidjan, Côte d’Ivoire, running 2018.
Methodology: Two references were used for the validation of the analytical method, namely the French standard NF V 03-110 and the ISO directive ISO/DIS/15753 applicable in Liquid Chromatography. The PAH contents of some samples collected from different stores were then determined.
Results: From the data, a significant regression chart was recorded for the PAH detection graphs. All the analysis exhibited good linearity with significant correlation coefficients (R²˃ 0.99). The relative standard deviations of the repeatability and reproducibility assays are below 3%, whereas standard additions of PAH are fully recovered, with percentages close to 100%.
Conclusion: Using this analytical method, kola nuts contamination by PAH have been determined with satisfactory.This analytical method could help in ensuring effective sanitary control at different critical points of kola nut distribution channel for promoting a good management of the toxicity concerns in such products.
Akpambang V, Izomoh A. Levels and sources of polycyclic aromatic hydrocarbons in urban soils of Akure, Nigeria. Chem. Sci. Int. J. 2016;17(3):1-10.
Albinet A, Leoz-Garziandia E, Budzinski H, Villenave E. Polycyclic aromatic hydro carbons (PAHs), nitrated PAHs and oxygenated PAHs in ambient air of the Marseilles area (South of France): Concentrations and sources. Science of the Total Environment, Elsevier. 2014; 384(1-3):1-24.
Haihua J, Qi W, Nana Z, Bo J, Xuliang Z, Zhihui B. Distributions and sources of Polycyclic Aromatic Hydrocarbons (PAHs) in soils around a chemical plant in Shanxi, China. Int. J. Environ. Res. Public Health. 2017;1198(14):1-19.
Sharma R, Chan WG, Seeman JI, Hajaligol M. Formation of low molecular weight heterocycles and Polycyclic Aromatic Compounds (PACs) in the pyrolysis of a amino acid. J. Anal. Appli. Pyrol. 2003;66:97-121.
Anietie M, Godwin E. Risk assessment of Polycyclic Aromatic Hydrocarbons PAHs in soils and tubers crops collected from Ikot Oborenyin, South-south Nigeria. Int. Res. J. Pure Appl. Chem. 2016;10(2):1-13.
Garnier R, Villa A, Chataigner D. Do the dangers of formaldehyde and toluene justify systematic substitution in hospitals and laboratories? Arch. Occup. Diseases Environ. 2011;72(3):252-255. French.
Shen H, Huang Y, Wang R, Zhu D, Li W, Shen G, Wang B, Zhang Y, Chen Y, Lu Y, Chen H, Li T, Sun K, Li B, Liu W, Liu J, Tao S. Global atmospheric emissions of polycyclic aromatic hydrocarbons from 1960 to 2008 and future predictions. Environ. Sci. Technol. 2013;47:6415-6424.
Jiao W, Wang T, Khim J, Luo W, Hu W, Naile J, Giesy J, Lu Y. Policyclic aromatic hydrocarbons in soils along the coastal and estuarine areas of the Northern Bohai and Yellow Seas, China. Environ. Monit. Assess. 2013;185(81):85-95.
Dobrinas S, Birghila S, Coatu V. Assessment of polycyclic aromatic hydrocarbons in honey and propolis produced from various flowering trees and plants in Romania. J. Food Compos. Anal. 2008;21:71-77.
Stanciu G, Birghila S, Dobrinas S. Residues of polycyclic aromatic hydrocarbons in different types of coffee. Scientific Study & Research. 2008;9(3): 325-330.
Walker C. Organic pollutants. An eco toxicological perspective. New York, CRC Press. 2009;414.
Meador J, Sommers F, Ylitalo G, Sloan C. Altered growth and related physiological responses in juvenile Chinook salmon (Oncorhynchus tshawytscha) from dietary exposure topolycyclic aromatic hydrocarbons. Can. J. Fish. Aquat. Sci. 2006;63:2364-2376.
EL-Saeid M, Sapp J. Distribution ratios of Polycyclic Aromatic Hydrocarbons (PAHs) in urban soils. J. Appl. Life Sci. Int. 2016;9(3):1-10.
Jarosław L, Ewa B. Polycyclic aromatic hydrocarbons content in contaminated forest soils with different humus types. Water Air Soil Pollut. 2018;229(204):1-8.
Seri Z. Draft action plan of the Appexco-ci 2013-2015. Center for Studies and Applied Research (CERA), Côte d’Ivoire. 2012;50.
Deigna-M, Kouadio K, Konan N, Biego G. Diagnosis in production and post-harvest processing of nuts of Cola nitida (Malvaceae) in Côte d’Ivoire. J. Agric. Ecol. Res. Int. 2016;9(2):1-11.
Niemenak N, Effa O, Lieberei R, Omokolo N. Purine alkaloids and phenolic compounds in three Cola species and Garcinia kola grown in Cameroon. S. Afr. J. Bot. 2008;74:629-638.
Nyamien Y, Coulibaly A, Belleville M, Petit E, Adima A, Biego G. Simultaneous determination of caffeine, catechin, epicatechin, chlorogenic and caffeic acid in Cola nitida dried nuts from Côte d’Ivoire using HPLC. Asian J. Biotech. Bioresour. Technol. 2017;1(2):1-7.
Adeosun S, Adejobi K, Famaye A, Idrisu M, Ugioro O, Nduka B. Combined effect of kola testa based organic manure and NPK fertilizer on soil, leaf chemical composition and growth performance of kola (Cola nitida). Res. J. Agric. Environ. Management. 2013;2(7):183-189.
Dah-Nouvlessounon D, Adoukonou-Sagbadja H, Diarrassouba N, Adjanohoun A, Baba-Moussa F, Sezan A, Baba-Moussa L. Indigenous knowledge and socio-economic values of three kola species (Cola nitida, Cola acuminata and Garicnia kola) used in Southern Benin. Eur. Sci. J. 2015;11:206-227.
Ajai I, Ochigbo S, Jacob O, Ndamitso M, Abubakar U. Proximate and mineral compositions of different species of kolanuts. Eur. J. Applied Eng. Sci. Res. 2012;1:44-47.
Kouadio R, Biego H, Nyamien Y, Konan Y, Konan C, Coulibaly A, Sidibe D. Validation of efficiency method for heavy metals determination in kola nuts (Cola nitida Schott & Endl.) from Côte d’Ivoire. Asian J. Adv. Res. Reports. 2019;6(3):1-9.
IARC: International Agency for Research on Cancer. Monographs on the evaluation of carcinogenic risks to humans. Some Non-Heterocyclic Polycyclic Aromatic Hydrocarbons and Some Related Exposures. Lyon, France; 2010.
French Association of Standardization. Analysis of agricultural and food products: Intralaboratory validation procedure for an alternative method compared to a reference method. Paris: Edition AFNOR, French. 1998;40.
International Standard Organisation (ISO). Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons. Geneve: ISO 15753. 2004;21. French.
Ake A, Biego G, Sess A, Koffi K, Kouame P, Bonfoh B, Akpagni H, Ausset E. Validation of a method for the quantification of polycyclic aromatic hydrocarbons in fish. Eur. J. Sci. Res. 2012;74:69-78.
Food and Agriculture Organization. Validation of analytical methods for food control. Vienna: Report of joint FAO/IAEA Expert Consultation. 1997;18.
American Public Health Association. Standard methods for the examination of water and wastewater. Washington DC: Franson M. A. H. 17th edition. 2005;72.
Juhasz A, Naidu R. Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: A review of the microbial degradation of benzo[a]pyrene. Int. Biodeter. Biodeg. 2000;45:57-88.
Food and Agriculture Organization (FAO). Thirty-seventh session of the Codex Alimentarius Commission on Contamination of Food with Polycyclic Aromatic Hydrocarbons (PAH), Rome, Italy. 2005;11.
Hoenner V. Assessment of pollution by PAHs and heavy metals in Alsace. Master Chemistry and Biology: Analytical Aspects, University of Louis Pasteur Strasbourg. 2006;43. French.
Airparif. Air pollution with benzo (a) pyrene and other polycyclic aromatic hydrocarbons (PAHs). Association of air quality monitoring in Ile-de-France. 2013;48. French.
Byambaa B, Yang L, Matsuki A, Nagato E, Gankhuyag K, Chuluunpurev B, Banzragch L, Chonokhuu S, Tang N, Hayakawa K. Sources and characteristics of polycyclic aromatic hydrocarbons in ambient total suspended particles in Ulaanbaatar City, Mongolia. Int. J. Environ. Res. Public Health. 2019;1-16.
Léotz-Gartziandia. Sampling and analysis of Polycyclic Aromatic Hydrocarbons (PAH) and oxygenated PAH in diesel exhaust and ambient air. Polycycl. Aromat. Comp. 2000;20:245-258.
Costes J, Druelle V. Polycyclic aromatic hydrocarbons in the environment: Rehabilitation of former industrial sites. Rev. Inst. Franç. Pétr. 1997;52(4):425-437.