Production of Nigerian Yoghurt Using Lactic Acid Bacteria as Starter Cultures

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S. Aforijiku
S. M. Wakil
A. A. Onilude


Aim: This work was carried out to investigate the influence of Lactic Acid Bacteria (LAB) on organoleptic quality and proximate composition of yoghurt, and viability of starter cultures in yoghurt.

Methods: The LAB starter cultures were selected based on their ability to produce diacetyl and lactic acid.

Results: Lactobacillus caseiN1 produced the highest quantity (2.72 g/L) of diacetyl at 48 hrs of incubation while Pediococcus acidilacticiG1 had the lowest amount (0.50 g/L). The pH of produced yoghurt ranged between 4.40 and 5.58 while the corresponding lactic acid contents ranged between 0.70 and 0.96 g/L. Yoghurt produced with cow milk inoculated with L. PlantarumN24 and L. BrevisN10 had the lowest pH (4.40) at significant level of P≤0.05. Yoghurt with mixed culture of L. PlantarumN24 and L. PlantarumN17 had the highest protein content (5.13%) while spontaneous fermentation (control) produced the least (0.48%). Yoghurt produced from cow milk inoculated with L. PlantarumN24 and L. PlantarumN17 was rated best with overall acceptability (9.0) during first day of storage while the commercial yoghurt (5.8) and spontaneous fermentation (6.8) had least overall acceptability at P≤0.05.

Conclusion: Yoghurt samples stored in refrigerator had more viable LAB counts for a period of 21 days while the samples stored at room temperature had a day count except for yoghurt produced with cow milk inoculated with L. plantarumN24 which retained its viability at the second day. The yoghurt produced with selected LAB starters are better than commercial yoghurt in terms of sensory properties, proximate composition, pH and viability.

Lactic Acid Bacteria (LAB), yoghurt, starter cultures.

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How to Cite
Aforijiku, S., Wakil, S. M., & Onilude, A. A. (2020). Production of Nigerian Yoghurt Using Lactic Acid Bacteria as Starter Cultures. Asian Food Science Journal, 16(3), 32-42.
Original Research Article


IS0, Yoghurt: Enumeration of characteristics microorganisms by colony count technique at 37°C. Ist Edn., International Standard Organisation, Brussels, Belgium; 2003.

Masci E. Bacteria and intestinal health in adult and pediatric population: Moving from the field of alternative medicine to evidence-based treatment. International Journal of Probiotics and Prebiotics. 2013; 8:1-4

Vasiee AR, Tabatabaei M, Yazdi F, Mortazavi A, Edalatian MR. Isolation, identification and characterization of probiotic Lactobacilli spp. from Tarkhineh. International Food Research Journal. 2014;21(6):2487-2492.

Mohammed S, Ahlgren JA, Horne D. Structural characterization and biological activities of an exopolysaccharide kefi ran produced by Lactobacillus kefiranofaciens WT-2B(T). Journal of Agricultural and Food Chemistry. 2016;52:5533-5538.

Brant RJ, Todd RK. Impact of genomics on the field of probiotic research: Historical perspectives to modern paradigms. A. V. Leeuwenhoek. 2014;106:141-156.

Rivera-Espinoza Y, Gallardo-Navarro Y. Non-dairy probiotic products. Food Microbiology. 2010;27:1-11.

Pérez-Chabela ML, Díaz-Vela J, Reyes-Menéndez CV, Totosaus A. Improvement of moisture stability and textural properties of fat and salt reduced cooked sausages by inoculation of thermotolerant lactic acid bacteria. International Journal of Food Properties; 2013.

Pérez-Chabela ML, Lara-Labastida R, Rodríguez-Huezo ME, Totosaus A. Effect of spray drying encapsulation of thermotolerant lactic acid bacteria on meat batters properties. Food Bioprocess and Technology. 2012;7:45-98

DOI: 10.1007/s11947-012-0865-y

A.O.A.C. Official Methods of Analysis. 15th Edn., Washington D.C.U.S.A: Association of Official Analytical Chemists; 1990.

ISBN 2-93 558 442-0

Nikolic M, Terzic-Vidojevic A, Jovcic B, Begovic Golic N, Topisirovic L. Characterization of lactic acid bacteria isolated from Bukuljac, a homemade goat’s milk cheese. International Journal of Food Microbiology 2008;122:162-170.

Odunfa SA, Oyewole OA. African fermented food. In Brain Wood JB. (Ed.) Microbiology of fermented food. 2nd edition Blackie Academic Professional. 1998;2

Ogunbanwo ST, Sanni AI, OniludeAA. Effect of bacteriocinogenic Lactobacillus spp. on the shelf life of fufu, a traditional fermented cassava product. World Journal Microbiology and Biotechnology. 2004;20: 57-68

Oyawoye OM, Oyawoye 0E, Bangbose AM, Danka SR. Effect of chemical preservatives on the shelf life of Nono. Applied Tropical Agriculture 1997;2:63-66.

Oyetayo VO, Osho B. Assessment of probiotic properties of a strain of Lactobacillus plantarum isolated from fermenting corn slurry (Ogi). Journal Food Agricultural and Environment. 2004;2(1): 132 -134 .

Ozer B, Kirmaci HA. Quality attributes of yoghurt and functional dairy products. In: Yildiz, F. Ed. Development and manufacture of yoghurt and other functional dairy products, CRC Press, Taylor and Francis group. 2010;229-265.

Wakil SM, Onilude AA. Microbiological and chemical changes during production of malted and fermented cerealed- legume weaning foods. Adavances in Food Sciences. 2009;31(3):139-145.

Achi OK, Akobor PI. Microbiological characterization of yam fermentation for elubo (yam). World Journal of Microbiology and Biotechnology. 2001;16: 3-7.

Nout MJR, Ecology of accelerated natural lacticfermentation of sorghum-based infant food formulas. International Journal of Food Microbiology. 1991;12: 217-224.

Adesokan IA, Ekanola YA, Fakorede SS, Oladejo OO, Odutola OL. Infuence of lactic starters on sensory properties and shelf life of wara-a Nigerian (unripened) soft cheese. Journal of Applied Bioscience 2009:13:714-719.

Adebayo-tayo BC, Onilude AA. Screening of Lactic Acid Bacteria Strains Isolated from Some Nigerian Fermented Foods for EPS Production. World Applied Sciences Journal. 2008;4: 741-747.

Wakil SM, Kazeem MO. Quality assessment of weaning food produced from fermented cereal-legume blends using starters. International Journal of Food Research. 2012;19(4):1679-1685.

Nwanyanwu CE, Alisi CS, Nweke CO, Orji JC. Cell surface properties of phenol utilizing bacteria isolated from petroleum refinery wastewater. Journal of Research Biology. 2012;2:383-391.