Extraction and Estimation of Protein from Ginger (Zingiber officinale) and its Interaction with Glucose Molecule
Asian Food Science Journal,
Aim: To evaluate the protein composition in the ginger rhizome and its interaction with glucose molecule.
Place and Duration of Study: Department of Biochemistry, Sokoto State University, Sokoto, Nigeria, between April 2021 and May 2021.
Methodology: Protein was extracted from the rhizome of ginger using 0.1 M phosphate buffer. The protein concentration of the sample was estimated using Biuret method while using xanthoproteic test, the presence of aromatic amino acids was ascertained. The crude protein sample was interacted with glucose using UV interaction study.
Results: The protein concentration of the sample (2 g) was found to be 1.702 mg/ml, it was identified that ginger rhizome contains aromatic amino acids. UV interaction study between the crude protein sample and glucose molecule showed an increase in absorbance at the range of 280 nm.
Conclusion: The interaction of ginger with glucose shows that it possesses a hypoglycemic effect.
- UV- interaction
How to Cite
Schauenberg P, Paris F. Guide to medicinal plants. Keats Pub-lishing, New Canaan CT, (1977)pp. 30-300.
Mao Q, Xu X, Cao S, Gan R, Corke H, Beta T, Li H. Bioactive compounds and bioactivities of ginger (Zingiber Officinale Roscoe). Foods. 2019;8: 185. Doi:10.3390/foods8060185.
Chrubasik S, Pittler MH, Roufogalis BD. “Zingiberisrhizoma: a comprehensive review on the ginger effect and efficacy profiles. Phytomedicine. 2005;12(9):684-701.
ElkhishinI A, Awwad IA. A study of the cardiovascular toxic effects of Zingiberofficinale (ginger) in adult male albino rats and its possible mechanism of action. Mansoura. J. Forensic Med. Clin. Toxicol. 2009;27(2):109-116. DOI:10.21608/mjfmct.2009.52913
Agu CS, Igwe JE, Amanze NN, Odumam O. Effect of oven drying on proximate composition of ginger. American journal of Engineering Research. 2016; 5(8):58-61.
Govindaraju C, Thomas J, Sudharsan MR. 'Chenthal' disease of cardamom caused by ColletotrichurngleosporoidesPenz and its management. Journal of Plantation Crops. 2003;255-259.
Wei C, Tsai Y, Korinek M, Hung P, El-Shazly M, Cheng Y, Wu Y, Hsieh T, Chang F. 6-Paradol and 6-shogaol, the pungent compounds of ginger, promote glucose utilization in adipocytes and myotubes, and 6-paradol reduces blood glucose in high-fat diet-fed mice. Int. J. Mol. Sci. 2017;18:168.
Mason PE, Lerbret A, Saboungi M, Neilson GW, Dempsey CE, Brady JW. Glucose interactions with a model peptide. Proteins. 2011;79(7):2224-2232.
Li Y, Tian XX. Liu T and Wang RT (2015). Association between whole blood viscosity and arterial stiffness in patients with Type 2 Diabetes mellitus. Endocrine 49 (1): 148-54.
Duke JA, Ayensu ES, Medicinal Plants of China. Annals of Epidemiology. 2001;29(1):156-163.
Barbarino E, Lourenço SO. An evaluation of methods for extraction and quantification of protein from marine macro- and microalgae. J. Appl. Phycol. 2005;7:447-460.
Chatterjea MN. Textbook of Biochemistry for Dental/Nursing/Pharmacy Students. 3rd ed. Jaypee Brothers Publishers; 2004.
Waddell W J. A simple UV spectrophotometric method for the determination of protein. The Journal of Laboratory and Clinical Medicine. 1956;48:311-314.
Nur MI, Widyarti S, Sumitro SB. The study of UV spectrum in interaction Astaxanthin and glycated Bovine serum albumin (Gly-BSA). Journal of Environmental Engineering and Sustainable Technology. 2019;6(1):23-29.
Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasalah U, Gopas J, NishigakI I. Antioxidants and human diseases. Clinica Chimica Acta. 2014;436:332- 347.
Abstract View: 77 times
PDF Download: 42 times