Asian Food Science Journal

Fermented milk is an essential commodity in Africa and beyond. Many techniques have been developed over time for the manufacture of different forms of yoghurt products. One of these Traditional methods includes back slopping). The advantages of this method include faster fermentation rates due to reduction in lag time, and subsequent production of relevant metabolites as well as allowing for a more reliable product formation on a consistent basis. The aim of this study was to better understand, the effects of back sloping on the microbial community as well as on the organoleptic characteristics of the yoghurts produced using the method. The model from this work could be used to study the dynamics of the microbial community associated with back-slopping practices and the understanding of possible associated defects in order to allow better control over the application of the method on commercial levels. We characterized the yoghurt produced from both microbial compositional study using culture-dependent morphological examinations on MRS, M17, Nutrient Agar and Potato dextrose Agar, as well as from organoleptic point of view. The results show that back sloping up to three-fold (batch) gave increasing acceptance but decreased afterwards. Acidification activity which determines proteolysis of casein for coagulation to take place also increased up to three folds. Residual lactose, syneresis and moisture content also decreased favourably by three fold order. Therefore, back sloping could be recommended on commercial level especially in the developing countries where facilities for consistent supply of pouched starter culture are limited because, aside the above mentioned advantages, this process also favours the growth of bacteria which release antimicrobial substances thereby ensuring the growth of the same species while reducing the growth of other organisms thereby preserving the products’ quality.