Improving the Antioxidant Activity of a Carbonated Lemon Soft Drink

Mohamed Yehia Sayed Ahmed

Chemistry of Flavour and Aroma Department, National Research Centre, Dokki, Cairo, Egypt.

Rasha Saad

Chemistry of Flavour and Aroma Department, National Research Centre, Dokki, Cairo, Egypt.

Fatma Shafik Abd El-Aleem *

Chemistry of Flavour and Aroma Department, National Research Centre, Dokki, Cairo, Egypt.

Shereen Nazeh Lotfy

Chemistry of Flavour and Aroma Department, National Research Centre, Dokki, Cairo, Egypt.

Hoda Hanem Mohamed Fadel

Chemistry of Flavour and Aroma Department, National Research Centre, Dokki, Cairo, Egypt.

*Author to whom correspondence should be addressed.


Abstract

The carbonated functional soft drinks are a new concept that provides health benefits for the consumers. The aim of this study was to improve the antioxidant activity of a carbonated lemon soft drink, while preserving its characteristic flavour, by blending the lemon oil (L) with oregano (Or) essential oil. The effect of different thickeners, usually used in formulation of the soft drinks, on release of the volatile compounds from simple model solutions was evaluated, separately. Blending lemon oil with oregano essential oil (1.5 µL Or /100 µL) improved the odour acceptability and increased the DPPH scavenging ability of lemon oil by 40%. Studying the effect of different thickeners indicated that the xanthan, Arabic gum and pectin at levels 0.05, 6.0 and 0.01 g/100 mL water, respectively, showed the highest release of limonene and citral, the most contributors to lemon aroma, from the simple model solutions. This finding was mainly correlated to the interaction between the thickeners. In view of this result a lemon soft drink was formulated. Carbonation of the soft drink improved the sensory characteristics, decreased the pH value, increased the acidity and showed insignificant changes (P > 0.05) in total soluble solids and total sugars.

Keywords: Lemon oil, oregano oil, carbonated soft drink, thickeners, sensory evaluation, antioxidant activity


How to Cite

Ahmed, M. Y. S., Saad, R., El-Aleem, F. S. A., Lotfy, S. N., & Fadel, H. H. M. (2023). Improving the Antioxidant Activity of a Carbonated Lemon Soft Drink. Asian Food Science Journal, 22(10), 22–36. https://doi.org/10.9734/afsj/2023/v22i10670

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References

Todaro A, Arena E, Timpone R, Parafati L, Proetto I, Pesce F, et al. Use of concentrated fruit juice extracts to improve the antioxidant properties in a soft drink formulation. Int J Gastro Food Sci. 2023; March 31:100649.

Available:https://doi.org/10.1016/j.ijgfs.2022.100649

Fadel HHM, Lotfy SN, Abd ElAleem FSh, Sayed Ahmed MY, Asfour MH, Abou Taleb SA, et al. Preparation and evaluation of a functional effervescent powder based on inclusion complexes of orange oil and β-cyclodextrin derivatives. J Essent Oil-Bear Plants. 2023;26(3):677–694. Available:http://dx.doi.org/10.1080/0972060X.2023.2236643

Brenna OV. Antioxidant Capacity of Soft Drinks. In Processing and impact on antioxidants in beverages. 2014;Jan 1:51-56. Available:http://dx.doi.org/10.1016/B978-0-12-404738-9.00006-4

Zhang H, Lou Z, Chen X, Cui Y, Wang H, Kou X, et al. Effect of simultaneous ultrasonic and microwave assisted hydrodistillation on the yield, composition, antibacterial and antibiofilm activity of essential oils from Citrus medica L. var. sarcodactyli. J Food Eng. 2019;244:126-135. Available:https://doi.org/10.1016/j.jfoodeng.2018.09.014

Otang WM, Afolayan AJ. Antimicrobial and antioxidant efficacy of Citrus limon L. peel extracts used for skin diseases by Xhosa tribe of Amathole District, Eastern Cape, South Africa. S Afr J Bot. 2016;102:46–49.

Available:https://doi.org/10.1016/j. sajb.2015.08.005

Bhatia H, Pal Sharma Y, Manhas RK, Kumar K. Traditional phytoremedies for the treatment of menstrual disorders in district Udhampur, J & K, India. India. J Ethnopharmacol. 2015;160:202–210.

Available:https://doi.org/10.1016/j.jep.2014.11.041

Clement YN, Baksh-Comeau YS, Seaforth CE. An ethnobotanical survey of medicinal plants in Trinidad. J Ethnobiol Ethnomed. 2015;11(67):1–28. Available:https://doi.org/10.1186/s13002-015-0052-0

Ameh SJ, Obodozie-Ofoeglbu O. Essential oils as flavours in carbonated cola and citrus soft drinks. Essential Oils in Food Preservation, Flavor and Safety. 2016;111-121.

Available:https://doi.org/10.1016/B978-0-12-416641-7.00011-0

Kosakowska O, Węglarz Z, Pióro-Jabrucka E, Przybył JL, Kraśniewska K, Gniewosz M, et al. Antioxidant and Antibacterial Activity of Essential Oils and Hydroethanolic Extracts of Greek Oregano (O. vulgare L. subsp. hirtum (Link) Ietswaart) and Common Oregano (O. vulgare L. subsp. vulgare). Molecules. 2021;26(4):988.

Available:https://doi.org/10.3390/molecules26040988

Yashin A, Yashin Y, Xia X, Nemzer B. Antioxidant Activity of Spices and Their Impact on Human Health: A Review. Antioxidants (Basel). 2017;6(3):1-18. Available:https://doi.org/10.3390/antiox6030070

Baricevic D, Bartol T. The biological/pharmacological activity of the Origanum Genus,in: Oregano, the genera Origanum and Lippia, ed. by Kintzios SE. Taylor and Francis, London. 2002;177-213.

Available:https://doi.org/10.1201/B12591-17

Singletary K. Oregano: Overview of the Literature on Health Benefits. Nut Today. 2010;45(3):129-138.

Available:http://doi.org/10.1097/NT.0b013e3181dec789

Bylaite E, Adler-Nissen J, Meyer AS. Effect of xanthan on flavor release from thickened viscous food model systems. J Agric Food Chem. 2005;53:3577–3583. Available:https://doi.org/10.1021/jf048111v

Fadel HHM, Taher MS, Gad AM, Kandil EM, Abd El-Aleem FSh. Flavour release from a banana soft drink complex model system: Evaluation of the efficiency of different adsorbents for trapping the released volatiles during storage. Int J Food Prop. 2015;18(4):796–807. Available:https://doi.org/10.1080/10942912.2014.911310

Mirhosseini H, Salmah Y, Nazimah SAH, Tan CP. Solid-phase microextraction for headspace analysis of key volatile compounds in orange beverage emulsion. Food Chem. 2007;105(4):1659–1670.

Available:https://doi.org/10.1016/j.foodchem.2007.04.039

Mirhosseini H, Tan CP, Hamid NSA, Yusof S. Effect of Arabic gum, xanthan gum and orange oil on flavor release from diluted orange beverage emulsion. Food Chem. 2008;107(3):1161–1172.

Available:https://doi.org/10.1016/j.foodchem.2007.09.043

Park SJ, Nurika I, Suhartini S, Cho WH, Moon KD, Jung YH. Carbonation of not from concentrate apple juice positively impacts shelf life. LWT- Food Sci Technol. 2020;134:110-128. Available:https://doi.org/10.1016/j.lwt.2020.110128

Ranganna S. Handbook of analysis and quality control for fruit and vegetable products, 6th edn. Tata McGraw-Hill Publishing Co. Ltd, New Delhi. 2000;652.

Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem. 1995;43:27–32. Available:http://dx.doi.org/10.1021/jf00049a007

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231–1237.

Available:https://doi.org/10.1016/s0891-5849(98)00315-3

Fadel HHM, El-Ghorab AH, Hussein AMS, El-Massry KF, Lotfy SN, Sayed Ahmed MY, et al. Correlation between chemical composition and radical scavenging activity of 10 commercial essential oils: Impact of microencapsulation on functional properties of essential oils. Arab J Chem. 2020;13(8):6815–6827.

Available:https://doi.org/10.1016/j.arabjc.2020.06.034

Hansson A, Andersson J, Leufvén A. The effect of sugars and pectin on flavour release from a soft drink-related model system. Food Chem. 2001;72(3):363- 368. Available:https://doi.org/10.1016/S0308-8146(00)00243-0

Nahon DF, Navarro y Koren PA, Roozen JP, Posthumus MA. Flavor release from mixtures of sodium cyclamate, sucrose, and an orange aroma. J Agric Food Chem. 1998;46:4963–4968. Available:https://doi.org/10.1021/jf980679e

Darwish AMG, Soliman TN, Elhendy HA, El-Kholy WM. Nano-encapsulated iron and folic acid-fortified functional yogurt enhance anemia in albino rats. Front Nut. 2021;8:1-23. Available:https://doi.org/10.3389/fnut.2021.654624

AOAC. Official Methods of Analysis, Association of Official Analytical Chemists. 15th edn. Arlington, VA, USA; 1990.

Larmond E. Laboratory Methods for Sensory Evaluation of Food. Research Branch, Canada Department of Agriculture, Publication No. 1637. 1977;19-63.

Cozzolino D, Cynkar WU, Shah N, Dambergs RG, Smith PA. A brief introduction to multivariate methods in grape and wine analysis. Int J Wine Res. 2009;1:123–130. Available:https://doi.org/10.2147/ IJWR.S4585

Aguilar-Hernández MG, Sánchez-Bravo P, Hernández F, Carbonell-Barrachina AA, Pastor-Pérez JJ, Legua P. Determination of the volatile profile of lemon peel oils as affected by rootstock. Foods. 2020; 9(2):241-247.

Available:https://doi.org/10.3390/foods9020241

Sedlaříková J, Doležalová M, Egner P, Pavlačková J, Krejčí J, Rudolf O, et al. Effect of Oregano and Marjoram Essential Oils on the Physical and Antimicrobial Properties of Chitosan Based Systems. Int J Polym Sci. 2017;2017:1-12. Available:https://doi.org/10.1155/2017/2593863

Wu Z, Li H, Yang Y, Zhan Y, Tu D. Variation in the components and antioxidant activity of Citrus medica L. var. sarcodactylis essential oils at different stages of maturity. Ind Crops Prod. 2013; 46(3):311–316.

Available:https://doi.org/10.1016/j.indcrop.2013.02.015

Al-Fatimi M. Volatile constituents, antimicrobial and antioxidant activities of the aerial parts of Origanum majorana L. from Yemen. J Pharm Res Int. 2018;23(4): 1-10. Available:https://doi.org/10.9734/JPRI/2018/35932

Kulisic T, Radonic A, Katalinic V, Milos M. Use of different methods for testing activity of oregano essential oil. Food Chem. 2004; 85(4):633–640. Available:https://doi.org/10.1016/j.foodchem.2003.07.024

Buljeta I, Pichler A, Ivić I, Šimunović J, Kopjar M. Encapsulation of Fruit Flavor Compounds through Interaction with Polysaccharides. Molecules. 2021;26(4): 4207. Available:https://doi.org/10.3390/molecules26144207

Crank J. The mathematics of diffusion, 2nd edn. Oxford University Press, London. 1975;69-88.

Boland AB, Buhr K, Giannouli P, Van Ruth SM. Influence of gelatin, starch, pectin and artificial saliva on the release of 11 flavour compounds from model gel systems. Food Chem. 2004;86(3):401-411.

Available:https://doi.org/10.1016/j.foodchem.2003.09.015

Schober AL, Peterson DG. Flavor Release and Perception in Hard Candy: Influence of Flavor Compound−Compound Interactions. J Agric Food Chem. 2004; 52(9):2623–2627.

Available:https://doi.org/10.1021/jf0353133

Secouard S, Malhiac C, Grisel M, Decroix B. Release of limonene from polysaccharide matrices: Viscosity and synergy effect. Food Chem. 2003;82(2): 227–234.

Available:https://doi.org/10.1016/S0308-8146(02)00518-6

Yang X, Zhu L, Chen Y, Bao B, Xu J, Zhou W. Controlled hydrophilic/hydrophobic property of silica films by manipulating the hydrolysis and condensation of tetraethoxysilane. Appl Surf Sci. 2016;376: 1–9.

Available:http://doi.org/10.1016/j.apsusc.2016.02.068

Friel EN, Linforth RST, Taylor AJ. An empirical model to predict the headspace concentration of volatile compounds above solution containing sucrose. Food Chem. 2000;71(3):309-317.

Available:https://doi.org/10.1016/S0308-8146(00)00190-4

Starzak M, Peacock SD, Mathlouthi M. Hydration number and water activity models for the sucrose-water system: A Critical Review. Crit Rev Food Sci Nutr. 2000;40(4):327-367. Available:http://doi.org/10.1080/10408690091189185

Arora S, Aggarwal P. Effect of method of preservation of pulp on the quality of carbonated and noncarbonated beverages prepared from peach fruit. J Food Qua. 2009;32(6):695-708.

Available:https://doi.org/10.1111/j.1745-4557.2009.00277.x

Sharma PC. Studies on fortification and carbonation juice beverages. Ph D thesis (Food Technol.), Agricultural University, Ludhiana, India. 1996;220.

Verma S. Fruit based carbonated soft drink for nutritional security and value chain development. Int J Curr Microbiol App Sci. 2018;7(11):3084-3095. Available:https://doi.org/10.20546/ijcmas.2018.711.354

Balaswamy K, Pamidighantam PR, Nagender A, Akula S. Preparation of sour grape (Vitis Vinifera) beverages and evaluation of their storage stability. J Food Process Technol. 2011;2(3):1-4.

Available:https://doi.org/10.4172/2157-7110.1000116

Ilamaran M, Amutha S. Effect of total soluble solids and CO2 pressure on physico-chemical and sensory qualities of carbonated banana and sapota beverage. J Food Sci Technol. 2007;44(2):178- 182.